Tobacco mutants with reduced microtubule dynamics are less susceptible to TMV

OUKO, MAURICE; SAMBADE, ADRIAN; BRANDNER, KATRIN; NIEHL, ANNETTE; PEÑA, EDUARDO; AHAD, ABDUL; HEINLEIN, MANFRED; NICK, PETER

PLANT JOURNAL

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2010 vol. 62 p. 829 - 929

0960-7412

A panel of seven SR1 tobacco mutants (ATER1 to ATER7) derived via T-DNA activation tagging and screening for resistance to a microtubule assembly inhibitor, ethyl phenyl carbamate, were used to study the role of microtubules during infection and spread of tobacco mosaic virus (TMV). In one of these lines, ATER2, alpha-tubulin is shifted from the tyrosinylated into the detyrosinated form, and the microtubule plus-end marker GFP-EB1 moves significantly slower when expressed in the background of the ATER2 mutant as compared with the SR1 wild type. The efficiency of cell-to-cell movement of TMV encoding GFP-tagged movement protein (MP-GFP) is reduced in ATER2 accompanied by a reduced association of MP-GFP with plasmodesmata. This mutant is also more tolerant to viral infection as compared with the SR1 wild type, implying that reduced microtubule dynamics confer a comparative advantage in face of TMV infection.p, li { white-space: pre-wrap; }