Preoperative Metabolic Signatures of Prostate Cancer Recurrence Following Radical Prostatectomy

CLENDINEN, CHAEVIEN S.; GAUL, DAVID A.; MONGE, MARÍA EUGENIA; ARNOLD, REBECCA S.; EDISON, ARTHUR S.; PETROS, JOHN A.; FERNÁNDEZ, FACUNDO M.

JOURNAL OF PROTEOME RESEARCH

AMER CHEMICAL SOC

Lugar: Washington; Año: 2019 vol. 18 p. 1316 - 1327

1535-3893

Technological advances in mass spectrometry (MS), liquid chromatography (LC)separations, nuclear magnetic resonance (NMR) spectroscopy, and big data analytics have made possible studying metabolism at an ?omics? or systems level. Here, we applied a multi-platform (NMR+LC-MS) metabolomics approach to the study of preoperative metabolic alterations associated with prostate cancer recurrence. Thus far, predicting which patients will recur even afterradical prostatectomy has not been possible. Correlation analysis on metabolite abundances detected on serum samples collected prior to surgery from prostate cancer patients (n=40 remission vs. n=40 recurrence) showed significant alterations in a number of pathways, including amino acid metabolism, purine and pyrimidine synthesis, tricarboxylic acid cycle, tryptophan catabolism,glucose, and lactate. Lipidomics experiments indicated higher lipid abundances on recurrent patients for a number of classes that included triglycerides, lysophosphatidylcholines, phosphatidylethanolamines, phosphatidylinositols, diglycerides, acyl carnitines, and ceramides. Machine learning approaches led to the selection of a 20-metabolite panel from a single preoperative blood sample that enabled prediction of recurrence with 92.6% accuracy, 94.4%sensitivity, and 91.9% specificity under cross-validation conditions.