An insulin like-peptide, INS-3, bridges neural perception of stressors with intracellular defensive mechanisms in non-neuronal cells of C. elegans

VEUTHEY TANIA V; GIUNTI SEBASTIAN; MASSON CAMILA; DE ROSA MARIA JOSE; RAYES DIEGO

Congreso; XXXIII Reunión Anual. de la. Sociedad Argentina de Investigación en Neurociencias; 2018

Multicellular organisms coordinate the systemic response to stress. We have shown that in C. elegans the acute-stress response activates neurons that release tyramine (TA), the invertebrate analog of adrenaline/noradrenaline. TA stimulates the DAF-2/Insulin/IGF-1 pathway and precludes the nuclear translocation of the DAF-16/FOXO transcription factor through the activation of an adrenergic-like receptor TYRA-3 in the intestine. In contrast, environmental long-term stressors reduce TA release allowing the induction of FOXO-dependent cytoprotective genes. However, how the insuline and tyraminergic pathway are linked is unknown. We here found that genetic silencing of an insulin like-peptide (ILP) (INS-3) increases the resistance to thermal and oxidative stress, reaching levels similar to tdc-1 (incapable of synthetizing TA) and tyra-3 null mutants. Moreover, unlike wild type animals, exogenous TA does not impair oxidative or thermal stress resistance. In addition, double null mutants between TA- deficient and ILPs null mutants (tdc-1 or tyra-3 with ins-3 or 7) showed levels of stress resistance similar to those found in INS-3 single null mutants, suggesting genetic interaction. Intestinal expression of INS-3 rescues the resistance phenotype of INS-3 null mutants to wild-type levels. We proposed that TA released form the nervous system promotes intestinal release of ILPs, which activate DAF-2 in other cells, inhibiting the systemic stress response mediated by DAF-16/FOXO.