Murine splenic ultrastructure in animal models with haemolytic anemia

DANNA CECILIA; GATTI CJ; GIAMBELLUCA M; CALVO NATALIA; VEUTHEY TANIA V; MARTA ELENA ROQUE

Congreso; International/ICLAS Regional Scientific Meeting: Advances in the Care and Use of Laboratory Animals; 2004

In oxidative injury caused by hemolytic drugs, splenic phagocytosis is increased by erythrocyte cytoskeleton protein alteration, lipid peroxidation, and methahemoglobinemia. The objective of this study was to assess, by means of scanning electron microscopy (SEM) , the morphological changes ocurring in the spleen structure of mice in an Hemolitic Anemia Model. Anemia was induced in female mice (CF 1) using phenilhydrazine (60 mg/kg) on days 0 and 2 of the protocol. The erythropoietic recovery was determined by conventional hematological parameters (days: 0,4,7). The splenectomy was performed by abdominal incision (days:0,4,7). The splenic tissue was fixed using glutaraldehide. SEM showed echinocytes (Heinz bodies) and erythrocytes that became sensitive (¨pitting¨) and significant changes in splenic tissue. Reticulocytary increase (46%, day:7) was consistent with hematocrit recovery (47%) and spleen hypertrophy. Three-dimensional imaging is useful to assess splenic tissue morphology in drug-induced oxidative injury.