Estrés oxidativo en un modelo de ratón

VEUTHEY TANIA V; GATTI CHRISTIAN; GIAMBELLUCA MIRIAM; D'ANNA MARIA CECILA; SANCHEZ MARIANELA; ROQUE MARTA ELENA

Tandil, Argentina

Congreso; X Congreso Argentino de Ciencias Morfológicas; 2006

Universidad Nacional del Centro - Facultad de Ciencias Veterinarias

Mean erythrocyte life depends on processes affecting its cellular metabolismand functionality. Oxidants such as Phenylhydrazine (PHZ) inducephenomena characterized by lipid peroxidation, Hb denaturalization, andpresence of phagocyted Heinz bodies, mainly by splenic macrophages. Thedetermination of the minimum effective dose of oxidative stress inducingPHZ in mice Strain CF1 was proposed in order to understand medullar andsplenic function in hemolytic anemia. Mice CF1 (43±3g) were groupedinto: G1-CONTROL: intraperitoneal (ip) physiological solution/300mL(Days:0,2 of the protocol) (n=9); G2-EXPERIMENTAL: PHZ ip between[0.5-24.8Mm] /300mL (Days:0,2) (n=72). Samples was obtained from G1and G2 (Days:0,4,6) (n=3). Anemia Evolution and Recovery: Hb and reticulocytes.Erythrocyte Oxidation degree: Heinz bodies by optical microscopy.Splenic Phagocytosis: Scanning electron microscopy. Medullar ErythropoiesisMagnitude: Optical microscopy. Statistical significance was setat P<0.05. Mice received free food and water. The [PHZ] dose-responsecurve vs. Hb/Reti/Heinz bodies showed: 1)[0.5-1.5mM] (Day:4): lack ofanemia; 2)[2.5-24.8mM] (Day:4): the degree of anemia was directly correlatedto [PHZ]. The hemolytic crisis took place on Day:4 [PHZ:24.8mM](Hb:9.8g/dL±0.9). Doses [2.5-8.2mM] induced mild anemia (Hb:10.8-13.4g/dL) Reticulocytes showed erythropoietic recovery (Day:6):46.6%±3.0[PHZ:24.8mM] and 18.62%±3.9 [PHZ:8.2mM], regarding the control group(1.5%±0.5) (P<0.05). The increase in Heinz bodies was parallel to [PHZ](Day:4): 96.0%±2.8 [24.8mM] and 72.5%±2.38 [8.2mM] regarding nonhemolyticdoses and G1. Medullar Quantification (Day:4): 1)Mild Anemia:16.5-20.5cel.x106/g; 2)Marked Anemia:5.4-11.9cel.x106/g; 3)CONTROL:8.5-11.5cel.x106/g (P<0.05) The degree of anemia depends on PHZ,the bone marrow assumes active erythropoiesis in mild anemia but not inmarked anemia, in which the spleen compensates volemia. Cellular indicatorsof oxidative damage efficiently valued the process with the presenceof Heinz bodies and their splenic phagocytosis.