Eritropoyesis en un modelo mamífero

VEUTHEY TANIA V; GATTI CHRISTIAN; D'ANNA MARIA CECILIA; BETINA GARCIA; PENNACCHIOTTI GRACIELA; MARTA ELENA ROQUE

Tandil, Argentina

Congreso; X Congreso Argentino de Ciencias Morfológicas.; 2006

Universidad Nacional del Centro - Facultad de Ciencias Veterinarias

The murine spleen restores volemia after hemolysis and there is evidenceon the contribution of the liver. The erythropoietic activity of the kidney,Êtheerythropoietin producing organ, remainsÊunknown. The purpose of our workwas to study the renal and hepatic erythropoietic involvement assessingthe cellularity characterizing erythron recovery. Mice CF1 (43±3g) weregrouped into: G1-CONTROL (n=9): 300mL intraperitoneal (ip) physiologicalsolution (Days:0,2); G2-EXPERIMENTAL (n=9): Phenylhydrazine ip(60mg/kg/300mL) (Days:0,2). In G1 and G2 retroorbital blood was obtained(Days:0,4,7) (n=3). Anemia evolution and recovery were determined byHb, HCT, and brilliant cresyl blue reticulocytes. The animals wereeuthanized by decapitation (days:0,4,7). Tissue dissection was performedby abdominal incision and then fixed using Bouin solution and 10% formalinand processed for H&E. Statistical significance was set at P<0.05.Mice received free food and water. Erythropoietic cellular islands weresemi-quantified by a pre-established score (10xfields): a)0-2 islands/10fields:+; b)3-7 islands/10fields:++; c)8-10 islands/10fields:+++; d)>10islands/10fields:++++. Renal and Hepatic Erythropoiesis: TransmissionElectronic Microscopy (TEM). Mean values (±DE) for GI and G2 (Day:0)were: Hb (15.5±0.7g/dL), HCT (48±2.5%), and reticulocytes (2.5±0.6%).In G2 (Day:4) Hb (9.8±0.9g/dL), HCT (31±2.0%), and reticulocytes(8.0±2.6%) showed acute hemolysis. While on Day 7, a clear compensationof anemia was observed: Hb (14.9±0.6g/dL), HCT (45.5±2.5%), andreticulocytes (46.6±3.0%) (P<0.05). G1 values remained unchanged duringthe experiment. The score showed: 1)G2-KIDNEY: Day0:+; Day4:+++;Day7:+++; 2)G2-LIVER: Day0:+; Day4:+++; Day7:++++; 3)G1-LIVERKIDNEY(Days:0,4,7):+. TEM showed undifferenciated erythrocytic cells.The presence of islands with erythropoietic morphology indicates that inthe acute hemolytic crisis in the kidney and liver they undertake a compensatingerythropoietic role, with a different degree of involvement.