INVESTIGADORES
RODRIGUEZ Andrea Paola
artículos
Título:
Frequent deletion of ING2 locus at 4q35.1 associates with advanced tumor stage in head and neck squamous cell carcinoma
Autor/es:
SILVIA S. BORKOSKY; MEHMET GUNDUZ; HITOSHI NAGATSUKA; LEVENT BEKIR BEDER; ESRA GUNDUZ; MAHMOUD AL SHEIKH ALI; ANDREA P. RODRIGUEZ; MEHMET ZEYNEL CILEK; SUSUMU TOMINAGA; NOBORU YAMANAKA; KENJI SHIMIZU; NORIYUKI NAGAI
Revista:
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
Editorial:
SPRINGER
Referencias:
Año: 2010 p. 509 - 518
ISSN:
0171-5216
Resumen:
Background Loss of heterozygosity (LOH) in the ING
family members has been shown in head and neck squamous
cell carcinoma (HNSCC) except for ING2. Like all
the other members of ING family, ING2, which is located
at chromosome 4q35.1, is a promising tumor suppressor
gene (TSG). In this study, we performed LOH analysis of
ING2 in HNSCC and compared it with clinicopathological
variables.
Materials and methods We performed LOH analysis in
DNAs from 80 paired of normal and HNSCC tissues, using
a speciWcally designed microsatellite marker on chromosome
4q35.1, which detects allelic loss of ING2. TP53
mutation analysis and its relationship with ING2 chromosomal
deletion were also performed in available 68 of
the samples. The correlation between LOH status and clinicopathological
characteristics was evaluated by using
statistical methods. The overall survival (OS) and disease
free survival (DFS) were also determined.
Results LOH was detected in 54.6% (30/55) of the informative
samples. Statistical signiWcance was obtained
between LOH and tumor (T) stage (P = 0.02), application
of radiotherapy and chemotherapy. Positive node status (N)
appeared to be the only independent prognostic factor for
both OS (P = 0.031) and DFS (P = 0.044).
Conclusions Our study showed allelic loss of 4q35.1 in
HNSCC. The high percentage of LOH suggests ING2 as a
candidate TSG in HNSCC. High LOH frequency was statistically
associated with advanced T stage, suggesting that
ING2 LOH might occur in late stages during HNSCC progression.