NEW SPOLIGOTYPES OF MICOBACTERIUM BOVIS ISOLATES OF PIGS FROM ARGENTINA

SOLEDAD BARANDIARAN; MARCELA MARTINEZ VIVOT; EDUARDO VICENTE MORAS; ANGEL CATALDI; MARTIN JOSE ZUMARRAGA

Bled

Congreso; 31 th ANNUAL CONGRESS OF THE EUROPEAN SOCIETY OF MYCOBACTERIOLOGY; 2010

EUROPEAN SOCIETY OF MYCOBACTERIOLOGY

New spoligotypes of Mycobacterium bovis isolates of pigs from Argentina. Soledad Barandiaran1,  Marcela Martínez Vivot1,  Eduardo Vicente Moras1,  Angel Cataldi2, Martín José Zumárraga2. 1Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Argentina. 2Instituto de Biotecnología, CICVyA-INTA-Castelar, Buenos Aires, Argentina. e-mail: sbaran@fvet.uba.ar; mzumarraga@cnia.inta.gov.ar Tuberculosis is a chronic disease affecting domestic and wild animals as well as human. Pigs are susceptible to M. tuberculosis, M. bovis and M. avium subsp. avium. In Argentina where bovine tuberculosis (BTB) has not been eradicated   the prevalence of M. bovis in pigs usually show the rate registered in the local cattle bovine population. The aim of this study was to identify spoligotypes of M. bovis isolates from pigs. One hundred thirty nine tuberculosis lesions were collected during the veterinary inspection in slaughterhouse during 2007-2009. The molecular typing of the M. bovis isolates were carried out using the reverse hybridization technique of Spoligotyping. Twenty eight different spoligotypes were identified and 121 (87%) isolates were grouped into 11 clusters. There were two main clusters designed SB0140 and SB0120 containing 76 (54.6%) and 17 (12.2%) isolates respectively.  This spoligotype was also the most frequently detected in cattle from Argentina. One hundred thirty isolates (93.5%) revealed identical spoligotypes to cattle, showing the source of infection. There were 9 spoligotypes (8 unique and one cluster with 2 isolates) exclusives of pigs because were not found neither in cattle nor other hosts from Argentina. Moreover, seven of them were not detected previously in the International data base of Sussex University. Five of seven (71.4%) of new spoligotypes were related with spoligotype SB0140 and 2 (28.6%) with SB0120 because they were differentiated by deletion of 2 to 6 spacers and 1 to 3 respectively. The finding of new spilogotypes of isolates from pigs could be due to the partial screening of the bovines with BTB in Argentina or the existence of M. bovis clones circulating wholly among pigs. On the other hand, the transmission between different hosts could provoke rearrangements of DR region showing different spoligotypes related to the previously described.  Key words: Mycobacterium bovis, spoligotyping, pigs.