Impact of bilirubin on the ABCC1 expression in brain parenchyma in vivo

ANDREA L. BERENGENO; SILVIA GAZZIN; CRISTINA BELLAROSA; M. CELESTE ROBERT; CLAUDIO TIRIBELLI

Mar del Plata

Congreso; LV Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2010

Sociedad Argentina de Investigación Clínica

IMPACT OF BILIRUBIN ON THE ABCC1 EXPRESSION IN BRAIN PARENCHYMA IN VIVO Berengeno A., Gazzin S., Bellarosa C., Robert M.C., Tiribelli C. Liver Research Center a.berengeno@csf.units.it Background: Severe hyperbilirubinemia in newborns and in patients with Crigler Najjar syndrome type I (CNSI) can results in the accumulation of unconjugated bilirubin (UCB)in specific regions of the brain (cerrebellum, basal ganglia and various brainstem nuclei) leading to chronic neurological damage. We demonstrated a strong down regulation of ABCc1 in the blood cerebrospinal fluid barrier of jaundiced Gunn rats (jj), the animal mod St, hippocampus: Hip) in hyperbilirubinemic (jj) animals and age-matched controls rats (JJ Gunn). Cerebral cortex (Cx) was used as control. Methods: Cx, Cll, St and Hip were examined in homozygous hyperbilirubinemic (jj) Gunn and age-matched controls (JJ) rats. Mrp1 mRNA level was assessed by Real Time-qPCR at P9, P17, P60 and protein expression by quantitative Western blot at P9. Results: Similar Mrp1 levels were found in Cx, Cll, St and Hip of JJ and jj rats at P9 and P17. In P60 animals, Mrp1 mRNa expression was unchanged in Cx,Cll and St; while an increase (p