Lipid metabolism in cancer cells: role of FABP5

KARINA ANDREA GARCIA; ANA VES-LOSADA; BETINA CÓRSICO; NATALIA SCAGLIA

Cordoba

Congreso; LII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; 2016

Cancercells show a metabolic reprograming towards macromolecular synthesis, requiredfor cellular growth. Fatty acid-binding proteins (FABPs) are intracellularproteins that bind hydrophobic ligands with high affinity. It has been proposedthat FABPs could transport theirs ligands to the different compartments withinthe cell. FABP5 is overexpressed in several types of cancers, but its function intumor metabolism is still unknown. We found that glucose and exogenous fatty acids alterFABP5 expression in A549 human lung adenocarcinoma cell. In addition, chemicalinhibition of de novo fatty acidsynthesis decreased FABP5 expression and cellular proliferation. Moreover, thesiRNA-mediated reduction of FABP5 in A549 cells impaired fatty acid uptake andproliferation compared to non targeting control siRNA-transfected cells. Thedistribution of exogenous fatty acids among different lipid species was,however, unchanged. Thus, our results suggest that FABP5 expression ismodulated by nutritional factors / metabolic conditions and it is essential forfatty acid uptake and proliferation in human lung cancer cells.