Genetic mapping of EST-SSRs, SSR and Indels to improve saturation of genomic regions in a previously developed sunflower map

PAOLA TALIA; VERÓNICA NISHINAKAMASU; HORACIO ESTEBAN HOPP; RUTH AMELIA HEINZ; NORMA PANIEGO

ELECTRONIC JOURNAL OF BIOTECHNOLOGY

UNIV CATOLICA DE VALPARAISO

Lugar: Valparaiso; Año: 2010 vol. 13 p. 1 - 19

0717-3458

In order to saturate a sunflower genetic map and facilitate marker-assisted selection (MAS) breeding for stress response, it is necessary to enhance map saturation with molecular markers localized in linkage groups associated to genomic regions involved in these traits. This work describes the identification and characterization of 1,134 SSR containing ESTs from unigenes available databases. Twelve of these functional markers as well as 41 public SSR markers were successfully localized in linkage groups, thus contributing to the saturation of specific regions on a reference genetic-linkage-map derived from a RIL mapping population from the cross between PAC2 x RHA266 lines. The enriched map includes 547 markers (231 SSR, 9 EST-SSR, 3 InDels and 304 AFLPs) distributed in 17 linkage groups (LG), spanning genetic size to 1,942.3 cM and improving its mean density to 3.6 cM per locus. As consequence, no gaps longer than 13.2 cM remain uncovered throughout the entire map, which increases the feasibility of detecting genes or traits of agronomic importance in sunflower. This map complements the previously published public map with a dense framework of AFLP, SSR, EST-SSR and EST-InDels markers loci in place for analysis of H. annuus (Kiani et al. 2007) suitable for the development of approaches that could be used to characterize traits which are difficult to manipulate through breeding programs.