Pathway analysis of cholesteryl ester accumulation effect on insulin response in hl-1 cardiomyocytes

ACTIS DATO, V; REY, FE; CHIABRANDO, GA; CESCHIN, DG

Congreso; SAIB-SAMIGE 2021; 2021

The cardiovascular disease (CVD) is a multisystem and multicellular pathology frequently associated with increased levels of aggregation-prone small LDL particles. Aggregated LDL (aggLDL) internalization promotes intracellular cholesteryl ester (CE) accumulation and the impairment of insulin response and glucose transporter type 4 (GLUT4) activity in cardiomyocytes. Lipid uptake was associated with cardiac dysfunction and cell death. Nevertheless, the link between CE accumulation and insulin response, cell function and viability in cardiomyocytes is not completely understood yet. The present study was conducted to determine intracellular pathways that are potentially regulated by cholesterol accumulation in heart. For this, we identify gene expression profiles by bulk transcriptome analysis in isolated RNA samples from cardiomyocyte-derived HL-1 cells exposed to aggLDL 100 µg/ml for 8 h and then stimulated or not with insulin 100 nM for 2 h. Considering a fold change (FC)>2 and p