Maltooligosaccharides production catalysed by cyclodextrin glycosyltransferase from Bacillus circulans DF 9R in batch and continuous operation

RODRIGUEZ GASTÓN, JORGELINA ANDREA; COSTA, HERNÁN; ROSSI, ANA LÍA; KRYMKIEWICZ, NORBERTO; FERRAROTTI, SUSANA ALICIA

PROCESS BIOCHEMISTRY - (Print)

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2012 vol. 47 p. 2562 - 2565

1359-5113

Cyclodextrin glycosyltransferase catalyses the synthesis of cyclodextrins from starch by intramoleculartransglycosylation. However, under certain conditions, the reaction can be directed towards the productionof linear dextrins, suppressing the synthesis of cyclodextrins. Thus, the starch acts as donormolecule and low molecular weight oligosaccharides act as acceptors in intermolecular transglycosylationreactions. In this paper the optimal conditions to transform starch into maltooligosaccharideswere determined employing glucose and maltose as acceptors and cyclodextrin glycosyltransferase fromBacillus circulans DF 9R as catalyst. Maltooligosaccharides production was optimised in a batch processreaching a yield of 60%. Besides, a continuous process was developed in a membrane reactor, increasingthe yield to 72% and multiplying eight times the productivity. In this way, it was possible to obtain ahigh purity product with higher efficiency, using a cyclodextrin glycosyltransferase. This is even moreadvantageous when compared with the processes currently used in industry which uses three enzymes.