Multimodal and non-linear optical microscopy applications in reproductive biology.

ADUR J; BARBOSA GISELA; PELEGATI VB; BARATTI MO; CESAR CL; CASCO VH; CARVALHO HF

MICROSCOPY RESEARCH AND TECHNIQUE (ONLINE)

Wiley Periodicals, Inc.

Año: 2016 vol. 79 p. 567 - 582

1097-0029

A plethora of optical techniques is currently available to obtain non-destructive,contactless, real time information with subcellular spatial resolution to observe cell processes.Each technique has its own unique features for imaging and for obtaining certain biologicalinformation. However none of the available techniques can be of universal use. For a comprehensiveinvestigation of biological specimens and events, one needs to use a combination of bioimagingmethods, often at the same time. Some modern confocal/multiphoton microscopes providesimultaneous fluorescence, fluorescence lifetime imaging, and four-dimensional imaging. Someof them can also easily be adapted for harmonic generation imaging, and to permit cell manipulationtechnique. In this work we present a multimodal optical workstation that extends a commerciallyavailable confocal microscope to include nonlinear/multiphoton microscopy and opticalmanipulation/stimulation tools. The nonlinear microscopy capabilities were added to the commercialconfocal microscope by exploiting all the flexibility offered by the manufacturer. The variouscapabilities of this workstation as applied directly to reproductive biology are discussed.