EFECTOS HISTOLÓGICOS DE UN AGONISTA DE GnRH EN OVARIOS DE FELINOS POSTNATOS: REPORTE PRELIMINAR

CARRANZA, ANA ., FAYA, MARCELA .,BATISTA, PABLO .,GOBELLO, CRISTINA .,BARBEITO, CLAUDIO

Santiago

Simposio; II Simposio Internacional de Reproduccion Animal; 2014

Universidad de Chile

COD ABSTRACT: MT375JR Area: Pequeños EFECTOS HISTOLÓGICOS DE UN AGONISTA DE GnRH EN OVARIOS DE FELINOS POSTNATOS: REPORTE PRELIMINAR (Histological effect of a GnRH agonist on the postnatal feline ovary: A preliminary report) Carranza, Ana 1,2., Faya, Marcela 1.,Batista, Pablo 1,2.,Gobello, Cristina 1,2 .,Barbeito, Claudio 3 ., 1 Laboratorio de Fisiología Reproductiva, Facultad de Ciencias Veterinarias (FCV), Universidad Nacional de La Plata (UNLP). 2 CONICET Argentina. 3 Cátedra de Histología y Embriología. Patología General, FCV, UNLP. The administration of GnRH agonists during the neonatal feline period may have a potential as a contraceptive agent in this species (Carranza et. al. 2014). The aim of this study was to describe the effect of the long -term release GnRH agonist, deslorelin acetate on the postnatal domestic cat ovary. Sixteen female cats were randomly assigned to one of the following treatment groups within the first 24 hours of birth: Deslorelin acetate 1.6 mg (Suprelorin; Virbac, France; DA; n=8) or Control (CO; n=8) and spayed immediately after puberty. The excised ovaries were weighed and processed routinely for histological assessment after hematoxylin -eosin stain. Follicles were classified as Bristol-Gould&Woodruff (2006). Micrographs were taken with a digital camera. The images were analyzed using the ImagePro Plus software. The number of primordial, primary, secondary and antral follicles, corpora lutea, and atretic follicles per square millimeter was determined using 20 images per animal taken with a X10 objective. Comparison between treatment s were carried out by Student test (P0.1). Primordial (461.4+/-3.0 vs. 1.074,3+/-117.5; P