POSTNATAL EXPOSURE TO A PROGESTIN DOES NOT PREVENT UTERINE ADENOGENESIS IN DOMESTIC CATS

M. LOPEZ MERLO, M. FAYA, P. DE LA SOTA, A. CARRANZA, C. BARBEITO, C. GOBELLO

Buenos Aires

Jornada; Jornada; V Jornadas de Jóvenes investigadores; 2015; 2015

Facultad de Ciencias Veterinarias UBA

Introduction: Neonatal PROGESTIN treatment in sheep and mice inhibited the development of UTERINE glands leading to a permanent aglandular UTERINE phenotype and producing infertility. An approach of this kind may provide a permanent contraceptive strategy with application in companion animal species for addressing overpopulation problems.Aim: The aim of the present study was to evaluate the effect of POSTNATAL administration of a PROGESTIN on UTERINE glands and fertility in DOMESTIC CATS. Secondly, the clinical safety of the treatment was also assessed. Materials and Methods: Ten POSTNATAL female kittens were assigned to one of these pharmacological protocols: Medroxyprogesterone acetate 10 mg SC (MPA; n=6) or Placebo: 0.2 cc corn oil SC (PLC; n = 4). All the animals were followed up until puberty (body weight, sexual behavior, clinical side effects) and then exposed to a fertile tomcat. Blood samples were taken for ovulation assessment, gestation was diagnosed by ultrasound and then the females underwent ovariohysterectomy. The uteri were examined macro and microscopically (H&E; ImagePro Plus). Differences between groups were carried out by Fisher Exact and Student´s t tests. Data were expressed as mean ± SEM and values P 0.1) of the 10 queens after the matings. The 7 ovulated queens also demonstrated to be fertile without differences between treatment groups (P > 0.1). No clinical side effects were observed (P > 0.1) except one pregnant MPA queen that presented an open cervix pyometra. Microscopic assessment of the uteri revealed the presence of UTERINE glands in all the cases. In the MPA treated queens the area occupied by UTERINE glands per m2 of endometrium (0.55±0.02 vs. 0.49±0.04; P >0.1) and the height of the UTERINE epithelium (m; 24.5± 0.7 vs. 24.4± 1.0; P >0.1) did not differ from the PCB group.Conclusion: It is concluded that a single POSTNATAL supraphysiological dose of medroxyprogesterone acetate did not ablate UTERINE ADENOGENESIS in DOMESTIC CATS. Furthermore, this treatment seemed to predispose to UTERINE disease without PREVENTion of fertility.enviar mensaje