Beneficial Effect of Moderate Exercise in Kidney of Rat after Chronic Consumption of Cola Drinks

CAO G, GONZÁLEZ J, MÜLLER A, OTTAVIANO G, AMBROSIO G, TOBLLI JE, MILEI J

PLOS ONE

PUBLIC LIBRARY SCIENCE

Lugar: San Francisco; Año: 2016 vol. 3 p. 1 - 16

1932-6203

AimThe purpose of this study was to investigate the effect of moderate intensity exercise on kidney in an animal model of high consumption of cola soft drinks.MethodsForty-eight Wistar Kyoto rats (age: 16 weeks; weight: 350 ? 400 g) were assigned to the following groups: WR (water runners) drank water and submitted to aerobic exercise; CR (cola runners) drank cola and submitted to aerobic exercise; WS (water sedentary) and CS (cola sedentary), not exercised groups. The aerobic exercise was performed for 5 days per week throughout the study (24 weeks) and the exercise intensity gradually increased during the first 8 weeks until it reached 20 meters / minute for 30 minutes. Body weight, lipid profile, glycemia, plasma creatinine levels, atherogenic index of plasma (AIP) and systolic blood pressure (SBP) were determined. After 6 month rats were sacrificed. A kidney histopathological score was obtained using a semiquantitative scale. Glomerular size and glomerulosclerosis were estimated by point-counting. The oxidative stress and pro-inflammatory status were explored by immunohistochemistry. A one way analysis of variance (ANOVA) with Tukey-Kramer post-hoc test or the Kruskal-Wallis test with Dunn?s post-hoc test were employed for statistics. A value of p < 0.05 was considered significant.ResultsAt 6 month, an increased consumption of cola soft drink were showed in CS and CR compared with water consumers (p