Insulin Receptor Expression In Peripheral Blood Mononuclear Cells As An Early Biomarker For Glucose Metabolism And Adiposity In Mother-child Dyads

MALPELI, A.; FASANO, MARÍA VICTORIA; ORELLANO, LAURA ; MAZZIOTA, LUCÍA; ROCHA, DANIELA; FOTIA LUCRECIA; MENDEZ IGNACIO; SALA, MARISA; OBREGON, PABLO; TOURNIER ANDREA; DISALVO LILIANA; VAREA, ANA; ANDREOLI M. F.

Toronto - Virtual

Congreso; Insulin 100; 2021

Universidad de Toronto

Rationale: early detection and prevention of diabetes and obesity is needed. Peripheral blood mononuclear cells (PBMC) are a source of molecular biomarkers reflecting metabolic status. Objective: to search associations between anthropometric and glucose homeostasis variables, including insulin receptor (InsR) gene expression in PBMC, in mothers with pregestational normal-weight (NW, n=31) and overweight/obesity (OW/OB, n=31) at 10 days postpartum and their children at 6 months of age. Findings: glucose, insulin, HOMA index and milk insulin did not differ between NW and OW/OB mothers. InsR gene expression was lower in OW/OB mothers and negatively correlated with pregestational body mass index (BMI) (r=-0.51, p=0.011) and with children zBMI at 10d (r=-0.40, p=0.049). Anthropometric variables, insulin and InsR did not differ between children from NW or OW/OB mothers. In mothers and children, InsR negatively correlated with adiposity (r=-0.69, p< 0.001 and r=-0.74, p=0.009, respectively), whereas insulin positively correlated with adiposity (r=0.35, p=0.006 and r=0.59, p=0.016, respectively). Conclusions: In the early postpartum, InsR gene expression in PBMC is a sensitive biomarker of glucose metabolism in mothers with OW/OB without insulin resistance. Adiposity correlated moderately with plasma insulin and strongly with InsR. PBMC are useful for early detection of metabolic disorders.