CLA prevents alterations in glycolytic metabolites induced by a high fat diet

ANDREOLI, M. F.; MARTINELLI, M.I.; SCALERANDI, MARÍA V; FARIÑA A. C.; WILLINER, MARÍA R.; BERNAL, CLAUDIO

EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY

WILEY-V C H VERLAG GMBH

Lugar: Weinheim; Año: 2012 vol. 114 p. 718 - 725

1438-7697

Conjugated Linoleic Acid has been reported to have beneficial and controversial effects on glucose and lipid metabolism. Besides, high fat diets induce alterations in liver and muscle lipid deposition, which could be associated with anomalous glucose utilization. Therefore, our aim was to evaluate whether the intake of Conjugated Linoleic Acid could prevent alterations in glycolytic intermediate metabolites and glycogen deposition induced by high fat diet. For this purpose, growing mice were fed a control diet (7% corn oil), a high fat diet (20% corn oil), or a high fat diet containing 17% corn oil + 3% Conjugated Linoleic Acid for 30 days. Liver and muscle glucose intermediate metabolites and glycogen were assessed. Liver glycolysis was inhibited by high fat, reflected by a decreased flow of substrates through phosphofructokinase-1 linked to elevated citrate. Conjugated Linoleic Acid at high fat diet prevented these alterations while increasing the lactate and glycogen synthesis. In muscle, high fat diet emphasized the reduction of the flux through phosphofructokinase-1, without additional changes in total glycogen levels. In conclusion, dietary Conjugated Linoleic Acid partially prevented glycolytic pathway alterations in liver but not in muscle of mice fed a high fat diet, associated with adverse effects as sustained hyperglycemia and hepatic lactate accumulation.