INVESTIGADORES
TEN HAVE Arjen
artículos
Título:
Regulation of endopolygalacturonase gene expression in Botrytis cinerea by galacturonic acid ambient pH and carbon catabolite repression.
Autor/es:
WUBBEN JP, TEN HAVE A, VAN KAN JAL AND VISSER J
Revista:
CURRENT GENETICS
Referencias:
Año: 2000 vol. 37 p. 152 - 157
ISSN:
0172-8083
Resumen:
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The phytopathogenic fungus Botrytis
cinerea
produces a set of endopolygalacturonases (endoPGs)
which
are involved in the enzymatic degradation of
pectin in plant cell
walls. The endoPGencoding genes of
B. cinerea are di€erentially
expressed when the fungus is
grown in liquid culture on di€erent
carbon sources. A
basic constitutive expression level was observed
for two
genes, Bcpg1 and Bcpg2, which encode basic isozymes.
Galacturonic acid was shown to induce the expression of
Bcpg4 and
Bcpg6. Low pH of the culture medium re
sulted in induced expression
of the Bcpg3 gene. Ex
pression of the Bcpg5 gene was inducible;
however the
inducing factors could not be identi®ed. Finally,
galac
turonic acidinduced expression of the Bcpg4 gene was
repressed
by the presence of morefavourable carbon
sources, such as glucose
