EXPOSURE TO HEXACHLOROBENZENE INDUCES ENDOCRINE ALTERATIONS ASSOCIATED WITH ENDOMETRIOSIS PROGRESSION

CHIAPPINI FLORENCIA; CEBALLOS LEANDRO; OLIVARES CARLA; BASTON JUAN IGNACIO; PONTILLO CAROLINA; MIRET NOELIA; FARINA MARIANA; SINGLA JJ; MERESMAN G ; RANDI ANDREA

Congreso; Reunión anual de Sociedades Biocientíficas 2019 LXIV Reunión Anual de la SAIC; 2019

Endometriosis is a chronic illness hormone-dependent which is defined by the presence of endometrial tissue outside the uterus. The endocrine disrupting chemicals may be involved in the development and progression of disease. Hexachlorobenzene (HCB) is a pesticide that acts as an endocrine disruptor modulating the hormonal signaling. Aberrant expression of estrogen and progesterone receptors (ER, PR) has been associated with progression of endometriosis, which is referred to as estrogen-dependent and progesterone-resistant. Aromatase is the key enzyme in the estrogen biosynthesis and it is essential for establishment and growth of endometriosis lesions. Previous results showed that HCB induces cell migration and invasion of endometrial cells, increases ERalfa; and reduces PR levels in lesion and eutopic endometrium in rat model. Our aim was to evaluate the dependence of ER on migration (scratch motility assay) and invasion (transwell assay) in endometrial stromal cells (T-HESCs), and to investigate the hormone receptor profile (WB) in T-HESCs and primary cultures of endometrial stromal cells from eutopic endometrium of control (CESC) women. Cells were exposed to HCB (0.005, 0.05, 0.5 and 5 μM) for 24h. Results showed that the pesticide enhanced cell migration (HCB 5 μM, p