How voluntary wheel running affects infarct size in thioredoxin overexpressing mice

PEREZ, V; FRANCO RIVEROS, V; OSSANI G; GODOY E; YANAJE CY; PAES DE LIMA, A; ARMANI, M; CASANOVA V; CICALE E; GRECO MC; BUCHHOLZ B; GELPI RJ; D' ANNUNZIO V

Congreso; ISHR - LA - Annual Meeting; 2021

International Society for Heart Research

Exercise training reduces myocardial injury caused by acute ischemia/reperfusion (I/R). Also, thioredoxin-1 (Trx1) maintains the cellular redox status and decreases the infarct size in I/R injury. However, it is not fully understood thioredoxin-1 role in exercised mice. The aim was to study if Trx1 is involved in the exercise cardioprotection mechanism. Wild type (Wt), transgenic overexpressing Trx1, and a dominant negative mutant (DN) of Trx1 mice were used and divided in exercised group (E) and sedentary group (S). Mice were placed in cages fitted with running wheels for 4 weeks. After the training period, mice hearts were subjected to 30min of I and 120min of R (Langendorff technique). The assessment of the infarct size was performed using triphenyl tetrazolium. Heart rate variation (∆HR%) and weight of mice, heart, and soleus were measured. Transverse muscle sections of soleus muscle were stained with H&E and the cross-sectional area (CSA) of fibers were measured. Data were expressed as mean±SEM and p