VOLUNTARY WHEEL RUNNING EFFECT IN TRX1-OVEREXPRESSION MICE SUBJECTED TO ISQUEMIA/REPERFUSION INJURY.

PEREZ, VIRGINIA; FRANCO RIVEROS, V; OSSANI G; EUGENIA GODOY; YANAJE CY; LEE AURORA; LEE BRIAN; GRECO MC; GIMENEZ S; BUCHHOLZ B; GELPI RJ; D' ANNUNZIO V

Mar del plata

Congreso; LXVII Reunion Anual de la Sociedad Agentina de Investigacion Clinica; 2022

Sociedad Argentina de Invetigacion Clinica

VOLUNTARY WHEEL RUNNING EFFECT IN TRX1-OVEREXPRESSION MICE SUBJECTED TO ISQUEMIA/REPERFUSION INJURY.Virginia Perez1,4, Verena Franco Riveros1, Georgina Ossani2,3,4, Eugenia Godoy1, Yohana Yanaje1,Verónica Casanova1, Eliana Cicale1, Carla Greco1, Néstor Lago3, Bruno Buchholz1,4, Ricardo J Gelpi,1,4, Verónica D´Annunzio1,4.1 Institute of Cardiovascular Physiopathology (INFICA), Department of Pathology, School of Medicine, University of Buenos Aires.2Laboratory of Experimental Medicine, Hospital Alemán, Buenos Aires, Argentina.3Centre of Experimental and Applied Pathology, Department of Pathology, School of Medicine, University of Buenos Aires.4 National Council of Scientific and Technical Research (CONICET), Argentina.Exercise training reduces myocardial injury caused by acute ischemia/reperfusion (I/R).Thioredoxin-1 (Trx1) maintains the cellular redox status and decreases the infarct size in I/R injury. However, it is not fully understood its role in exercise mice. The aim was to study if Trx1is involved in the exercise cardioprotection mechanism. Wild type mice hearts (Wt), transgenic mice hearts overexpressing Trx1, and a dominant negative mutant (DN) of Trx1 were used, mice were divided in exercise group (E) and sedentary group (S). Mice were placed in cages fitted with running wheels during 4weeks. After the exercise-training period,mice hearts were subjected to 30min of I and 120min of R (Langendorff technique). The assessment of the infarct size was performed using TTC. Also, heart rate variation (∆HR%) and mice, heart, and soleus weight was measured. Transverse muscle sections of soleus muscles were stained with H&E and the cross-sectional area (CSA) of myofibers were measure. Data were expressed as mean±SEM and p