Trypanosoma cruzi INFECTION AFFECTS ADIPOCYTE FUNCTION IN VIVO AND IN VITRO

GONZÁLEZ FB, ET AL

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Adipose tissue (AT) influences energy homeostasis and inflammation and also acts as Trypanosoma cruzi (Tc) reservoir. We recently showed that C57BL/6 mice infected with Tc displayed an exacerbated pro-inflammatory milieu associated with metabolic abnormalities like manifest hypoglycemia, hypoleptinemia and a markedly loss of AT mass, which may play a role in lethality. Probably, pro-inflammatory factors released during infection may be involved in lipolysis and also influence adipocyte function. In order to characterize AT wasting, we evaluate in vivo how Tc infection affects AT histology, features of inflammatory infiltrate and the expression of factors involved in inflammation, lipolysis and adipogenesis. Other series of studies were carried out in vitro using 3T3-L1 adipocytes exposed to plasmas derived from healthy (Co) and Tc-infected mice. Results: After 17 days post-infection (17 dpi), adipocyte number and size diminished [size(pixel2x105) media±SEM; Co=2.7±0.2; Tc=1.3±0.1, p