Sexual dimorphism of cellular and humoral immunity in experimental vaccines against Trypanosoma cruzi

BULFONI BALBI C, ; DINATALE B, ; PACINI MF, ; GONZÁLEZ F; VILLAR S, PROCHETTO E, FARRÉ C, CHAPO G, MARCIPAR, CABRERA, PÉREZ AR.

Congreso; Reunión anual de Sociedades de Biociencias SAIC.SAI.AAFE.NANOMED.AR; 2021

There is evidence that vaccine efficacy is affected by diverse parameterslike sex between other. Thus, the inclusion of both sexesin studies of immune function becomes increasingly imperative.Usually, vaccine research against T. cruzi (Tc) has been focused onantibody response evaluation. Thus, we aim to evaluate possiblevariations in humoral and cellular response induced after experimentalvaccine administration against Tc between BALB/c males(M) and females (F). Thus, mice (n=5/group) were immunized intranasally[3 doses, one every 2 weeks with 10μg of TS emulsifiedin ISPA (I) or c-di-AMP (A) adjuvants]. Controls were treated withsaline (S) or TS alone. Fifteen days after the last immunization, invivo cell-mediated (delayed hypersensitivity test -DHT-) and specifichumoral (ELISA) responses were assayed. Later, mice were orallychallenged with 3000 Tc (sub-lethal challenge). Parasitemia andclinical score were evaluated until day 100 post-infection (pi). In bothM and F, the DHT was higher in TS+I and TS+A groups comparedto their respective controls (p