IL-23 promotes IFN-gamma secretion on human NK cells and primes for IL-18 responsiveness

ZIBLAT A; IRAOLAGOITIA XL; SPALLANZANI RG; DOMAICA CI; TORRES NI; FUERTES MB; ZWIRNER NW

Congreso; LIX Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica y LXII Reunión Científica Anual de la Sociedad Argentina de Inmunología.; 2014

IL-23 is a member of the IL-12 family of cytokines involved in pro- and anti-tumoral responses. NK cells express IL-23 receptor but the effects of IL-23, mainly produced by dendritic cells (DCs), on human NK cells remain unknown. Previously, we demonstrated that IL-23 induced the secretion of IFN-gamma (IFN-g) but not cytotoxicity of human NK cells against Raji cells. Also, we showed that IL-23 induced a significant up-regulation of CD25 and had a priming effect for IL-18 induced secretion of IFN-g. Therefore, the aim of this work was to further study the role of IL-23 on human NK cells, and the mechanism and signaling pathways underlying the IL-23-induced production of IFN-g. We observed by flow cytometry that IL-23 induced a significant up-regulation of CD69 (p