Micronuclei and nuclear anormalities in anuran larvae from agroecosystems

POLLO FE; BABINI S; BIONDA CL; SALAS NE; MARTINO AL

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2013 vol. 37 p. 119 - 119

0327-9545

The pampean region has presented an important agricultural and livestock expansion, reducing the habitat quality and negatively impacting in anuran amphibians, which by its characteristics are bio-indicators of environmental quality. Our aim was to determine the frequency of micronuclei (MN) and nuclear abnormalities (NA) in erythrocytes of anuran that inhabit in environments with different alteration degrees in suburban area of Río Cuarto, Córdoba. The sampling was conducted at three sites: two agroecosystems (C1 and C2) and a site with a lower anthropic intervention (SM). In each site, five larvae of Bufo arenarum (B.a) and Leptodactilus latrans (L.l) between stages 30-40 were collected. Blood was taken from each larvae by cardiac puncture and a smear was prepared, which was stained with May Grunwal-Giemsa and observed microscope (1000X). The frequency of Mn and NA were calculated on 1000 erythrocytes. The results were B.a: C1: MN 1,5±1,2 and NA 2,8±3,72; C2: Mn 1,19±0,48 y AN 5,38±5,08; SM: MN 0,64±0,46 y NA 0,84±0,94; whereas for L.l: C1: MN 0,60±0,60 y AN 2,5±2,26; C2: MN 0,87±0,49 y NA 5,90±4,70; SM: MN 0,10±0,22 y NA 1,35±1,49. These results allow us to infer a relation between the alteration degrees of the environment with genetic abnormalities present in anurans, and presume the existence of genotoxic compounds in agricultural and livestock sites.