FISH AS BIOINDICATORS THE AQUATIC SYSTEMS

POLLO FE; SALAS NE ; MANCINI MA ; MARTINO AL

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2011 p. 235 - 235

0327-9545

The water quality of urban lake depend, among other causes, ac- tivities that take place in the lake and its drainage basin. Often, these ecosystems become important reservoirs of xenobiotics. The aim of this study was to determine the frecuency of micronuclei (MN) and nuclear abnormalities (NA) levels of fish from Villa Dálcar urban lake (33º 07? W, 64º 20? S, Río Cuarto, Argentina). The species studied were Cyprinus carpio, Cheirodon interrup- tus and Astyanax eigenmanniorum. We captured 98 specimens of three species, with values of 5,56 (± 1,42), 5,38 (± 0,26) and 5,72 (± 0,36) cm in total length. Blood samples were extracted by cutting the caudal peduncle, after anesthesia. The blood smears were fixed and subsequently stained with Giemsa and observed under a microscope with an increase of 1000X. The frequency of micronucleated erythrocytes (MN) and nuclear abnor- malities (NA) was calculated on 1000 red blood cells. The results were: C. carpio (0.05 ± 0.12 MN; 2.12 ± 1.88 NA), C. interruptus (0.04 ± 0.09 MN to 5.62 ± 6,32 NA) and A. eigenmanniorum (0.07 ± 0.22 MN; 8.77 ± 7.46 NA). These results allow us to infer the existence of genotoxic compounds in Villa Dálcar Lake. More detailed studies should be performed with the A. eigenmanniorum as it outlines a priori as a good ?sentinel? for the detection of xenobiotics.