In vitro antagonism of Trichoderma species against Fusarium tucumaniae and Fusarium virguliforme

SCANDIANI, M.M.; FAURA, A.; VARGAS, W.; LUQUE, A.; GONZALEZ-ANTA, G.

Pasadena, CA

Congreso; 2015 APS Annual Meeting; 2015

American Phytopathological Society

Soybean is the most important crop in Argentina, and it can be severely affected by different sudden death syndrome Fusarium causing species. The dominant specie is F. tucumaniae (Ft), followed by F. virguliforme (Fv). These pathogens infect soybean roots early after planting and seed treatments with fungicides or antagonistic organisms would be a good tool for an integrated disease management. To determine the potential antagonistic variation of isolates of Trichoderma, primarily T. koningiopsis and T. harzianum, were compared for the control of Ft and Fv. In vitro paired cultures of 27 isolates of Trichoderma were started simultaneously with Ft and Fv on PDA. The cultures were incubated at 22°C, 12 h fluorescent light-12hdarkness, during 15 days, and were scored for antagonism at 7 and 15 days, on a 1-5 scale, where 1: Trichoderma completely overgrew the pathogen and covered the entire medium surface, and 5: the pathogen completely overgrew the Trichoderma and ocuppied the plate surface. With Ft, 9 isolates of Trichoderma rated class 1. Of those 9, only 3 were highly antagonistic to the isolate of Fv. Both SDS Fusarium causing species developed inhibition zones against some strains of Trichoderma. The results show the potential SDS biocontrol by native soil Trichoderma spp.