INVESTIGADORES
VOLENTINI Sabrina Ines
congresos y reuniones científicas
Título:
OXIDATIVE TREATMENT INHIBITS THE GROWTH OF Penicillium expansum
Autor/es:
LAZARTE MA; CERIONI L; RAPISARADA VA; RODRÍGUEZ-MONTELONGO, L.; VOLENTINI SI
Lugar:
Tucuman
Reunión:
Congreso; VII CONGRESO ARGENTINO DE MICROBIOLOGÍA GENERAL SAMIGE DEL BICENTENARIO; 2011
Institución organizadora:
SAMIGE
Resumen:
Argentina is the main producer of apple in southern hemisphere. Blue mold
rot caused by Penicillium expansum is the most important postharvest
disease of apples, causing major economic losses. Synthetic fungicides are
used to control this disease, but the effectiveness of them has been seriously
compromise with the appearance of certain resistant biotypes of P.
expansum. Therefore, is desirable to find alternative methods to the
conventional fungicides for control of blue mold rot. In our laboratory, we
have standardized a sequential oxidative treatment (SOT), which is able to
inhibit the growth of Penicillium digitatum, P. italicum and Geotrichum
candidum, causal agents of citrus postharvest diseases. The SOT consists on
a first incubation with NaClO followed by a second incubation with H2O2 and
CuSO4. The combination of these compounds in the SOT generated a
synergistic effect. Here, we analyzed the effects of the oxidizing compounds
on P. expansum growth and conidia germination. We determined the MIC for
each compound and we established conditions for an oxidative treatment
against this fungal pathogen. Results shown that P. expansum was more
sensitive than P. digitatum and P. italicum, since in vitro growth was
completely inhibited by a brief incubation of 2 min with 5 ppm NaClO, 6 mM
CuSO4 and 100 mM H2O2. Moreover, when we analyzed the conidia
germination in lethal and sublethal conditions, we found a direct correlation
with conidia growth inhibition. Thus, we demonstrate that the oxidative
treatment could be used as an alternative methodology to eliminate P.
expansum conidia.