INVESTIGADORES
VOLENTINI Sabrina Ines
artículos
Título:
A critical phosphate concentration in the stationary phase maintains
Autor/es:
LICI A. SCHURIG-BRICCIO; MARIA R. RINTOUL; SABRINA I. VOLENTINI; RICARDO N. FARIAS; LAURA BALDOMA`; JOSEFA BADIA; LUISA RODRIGUEZ-MONTELONGO; VIVIANA A. RAPISARDA
Revista:
FEMS MICROBIOLOGY LETTERS
Editorial:
Blackwell Publishing
Referencias:
Lugar: England; Año: 2008 vol. 284 p. 76 - 83
ISSN:
0378-1097
Resumen:
Escherichia coli NADH dehydrogenase-2 (NDH-2) is a primary dehydrogenase in
aerobic respiration that shows cupric-reductase activity. The enzyme is encoded by
aerobic respiration that shows cupric-reductase activity. The enzyme is encoded by
NADH dehydrogenase-2 (NDH-2) is a primary dehydrogenase in
aerobic respiration that shows cupric-reductase activity. The enzyme is encoded by
ndh, which is highly regulated by global transcription factors. It was described that
the gene is expressed in the exponential growth phase and repressed in late
stationary phase. We report the maintenance of NDH-2 activity and ndh expression
in the stationary phase when cells were grown in media containing at least
37mM phosphate. Gene regulation was independent of RpoS and other transcription
factors described to interact with the ndh promoter. At this critical phosphate
concentration, cell viability, oxygen consumption rate, and NADH/NAD1 ratio
were maintained in the stationary phase. These physiological parameters gradually
changed, but NDH-2 activity remained high for up to 94 h. Phosphate seems to
trigger an internal signal in the stationary phase mediated by systems not yet
described.