Linfoma canino como modelo de estudio de linfoma no Hodgkin humano

M CALIRI; DANIELA M FERRÉ; M NIEVES; GORLA, N.B.M.

Mendoza

Otro; Tercer Simposio Internacional de Medicina Traslacional; 2023

UNCUYO

The spectrum of malignant lymphoid neoplasms in humans is similar to what is observed in canines (CF), preserving morphological similarities and biological behavior. Canine lymphoma is a relevant model of human non-Hodgkin lymphoma to define causality, progression, and the testing of new treatments; the advantage is that in this animal model, the disease occurs spontaneously. It is necessary to cytogenetically characterize the disease in CF, as little is known about possible chromosomal and gene rearrangements. The hypothesis of this project is that canine and human lymphomas exhibit specific and homologous chromosomal alterations (CAs).The aim of this study is to analyze CAs in canines with lymphomas and identify the involved chromosomal breakpoints.Lymphocyte cultures from 4 canines were performed using conventional cytogenetic techniques. Giemsa-stained chromosomal spreads were evaluated for 20 metaphases per animal to search for numerical and structural CAs: chromatid breaks and gaps (CTB and CTG), chromosome breaks and gaps (CSB and CSG), telomeric associations (AT), deletions, sticky metaphases, and aberrant mitoses. Five karyotypes per animal were assembled using G-banding. Fluorescence in situ hybridization (FISH) was performed with a centromeric probe for CF.The CAs per metaphase found were: CTB= 0.68±0.07; CSB= 0.70±0.07; AT= 0.16±0.05; CTG= 0.41±0.00; CSG= 0.55±0.07, sticky metaphases= 0.37±0.19. The chromosomes involved were numbers 8, 11, and 14; however more cases need to be studied to confirm these observations. FISH allowed for the precise orientation of chromosomes and obtaining 2n=78.The identification of chromosomal breakpoints involved in this hematological neoplasm will subsequently allow the use of genetic maps from both species to search for chromosomally concordant regions. A more comprehensive characterization of the canine disease serves a dual purpose: studying the rearrangements involved in canine neoplasms and accelerating the progress of knowledge about these diseases in both species.