Single cell transfection of human-induced pluripotent stem cells using a droplet-based microfluidic system

PÉREZ-SOSA, CAMILO; SANLUIS-VERDES, ANAHÍ; WAISMAN, ARIEL; LOMBARDI, ANTONELLA; ROSERO, GUSTAVO; GRECA, ALEJANDRO LA; BHANSALI, SHEKHAR; BOURGUIGNON, NATALIA; LUZZANI, CARLOS; PÉREZ, MAXIMILIANO. S.; MIRIUKA, SANTIAGO; LERNER, BETIANA

Royal Society Open Science

Royal Society Open Science

Año: 2022 vol. 9

Microfluidic tools have recently made possible many advances in biological and biomedical research. Research fields such as Physics, Engineering, Chemistry and Biology have combined to produce innovation in Microfluidics which has positively impacted on areas as diverse as nucleotide sequence, functional genomics, single-cell studies, single molecules assays, and biomedical diagnostics. Among these areas regenerative medicine and stem cells have benefited from Microfluidics due to these tools have had a profound impact on their applications. In the study, we present a high-performance droplet-based system for transfecting individual human-induced pluripotent stem cells (hiPSCs). We show that this system has great efficiency in single cellsand captured droplets, similar to other microfluidic methods and lower cost. We demonstrate that this microfluidic approach can be associated with the PiggyBac transposase-based system to increase its transfection efficiency. Our results provide a starting point for subsequent applications in more complex transfection systems, single-cell differentiation interactions, cell subpopulations, cell therapy, among other potential applications.