TBX20 OVEREXPRESSION INDUCES CELL PROLIFERATION AND ANGIOGENESIS IN VITRO

BAUZÁ MARÍA DEL ROSARIO; LÓPEZ AYELÉN EMILCE; SIMONIN JA; GONZÁLEZ GERMÁN; BELAICH MARIANO NICOLÁS; CUNIBERTI LUIS; CROTTOGINI ALBERTO; LOCATELLI PAOLA; OLEA FERNANDA DANIELA

Mar del Plata

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2019

Sociedad Argentina de Investigación Clínica

Introduction: In the last years, several therapies aimed at myocardial regeneration have been investigated to treat acute myocardial infarction (AMI). To reach this aim, the main approaches are to induce neovascularization (angiogenesis) and myocardial self-regeneration (myocardiogenesis).It has been shown that the overexpression of TBX20 transcription factor in transgenic mice induces cardioprotective effects, suggesting that the overexpression of TBX20 could be a therapeutic alternative for cardiac regeneration. Objectives: Our objective was evaluated the effect of the overexpression of TBX20 over cell proliferation and angiogenic induction in vitro. Materials and methods: Baculoviral vectors overexpressing human TBX20 (BvTBX20) and control vectors (BVNull) were generated. Then, H9c2 cells were transduced with these vectors using a MOI of 300. At 2 and 5 days post-transduction cell proliferation was evaluated by MTS assay and cell count. Supernatants from transduced cells were used to perform a tubulogenic assay in HMEC cells.Results: Cell proliferation rate was highest at 5 days post-transduction in the BvTBX20 group vs BvNull assessed by MTS assay (BvTBX20: 107±1; vs. Bvnull: 100±1 % cell proliferation, p