The potential of hypoxic preconditioning on the angiogenic profile of human adipose-derived muse cells

CASTILLO MG; BAUZÁ MR; GIMÉNEZ CS ; SIMONIN JA; CROTTOGINI AJ; OLEA FD; CUNIBERTI L.

CIUDAD DE BUENOS AIRES

Congreso; Reunión Conjunta de Sociedades de Biociencias.; 2017

Sociedades de Biociencias.

Multilineage-differentiating stress-enduring (Muse) cells are a novel type of nontumorigenic endogenous pluripotent-like stem cells that can be isolated from adult tissues. Mesenchymal stromal cells (MSCs) are of great interest as therapeutic agents for regenerative purposes. Several studies have focused on the beneficial effects of hypoxic preconditioning on MSC survival; however, their potential impact on Muse cells remains unclear. The hypoxia inducible factor-1 α (HIF-1α) is known to be a transcription factor that is strongly induced by hypoxia and one of its important functions is to promote angiogenesis. This is done via HIF-1α regulation of vascular endothelial growth factor (VEGF) transcription. VEGF is a major regulator of angiogenesis, which promotes endothelial cell migration. Our objective was to obtain Muse cells from primary cultures of subcutaneous human adipose tissue (AT), identify their pluripotency, and analyze angiogenic profile changes induced by hypoxic preconditioning. Muse cells were isolated from human female abdominal lipoaspirates using severe cellular stress procedure. At 6 days Muse-AT cells showed positive expression for pluripotency markers: Nanog, Oct4, and Sox2, as well as stage-specific embryonic antigen-3 (SSEA-3). Hypoxic preconditioning (HP) protocol (6hs, 1.5%O2) or normoxia condition (NC) was applied at 2 or 6 days after isolation. HIF-1α expression was decreased at day 2 (NC: 0.7±0.1 fold; and HP: 0.2±0.04 fold; p