HIF1A/HIF3A RELATION IN ACUTE MYOCARDIAL INFARCTION PROGNOSIS: IN SILICO ANALYSIS

NUÑEZ PEDROZO CN; PERALTA TM; CASTILLO MG; LOCATELLI P; OLEA FD; CROTTOGINI AJ; CUNIBERTI L

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAI&FAIC SAFIS 2022; 2022

SAIC, SAI, SAFIS

Objectives: The hypoxia-inducible factor-1 (Hif1) is a major regulator of the hypoxic response after acute myocardial infarction (AMI). It is known that mammalian hearts have limited regeneration capacity. Over the last years, molecular therapies have sought to enhance Hif1 expression to induce angiogenesis and cell survival. In the present research we evaluated the Hif1a/Hif3a relation in the transcriptomes of infarcted mice belonging to the regenerative (R) and non-regenerative (NR) evolutionary stages. Methods: The bulk RNA-Seq files (n=36) were retrieved from GEO (GSE142366). The heart samples were taken at 1.5, 3 and 7 days post-AMI for both neonatal (R) and infant mice (NR) and non-infarcted mice at equal times. The files were processed on UseGalaxy employing the FastQC/HISAT/ featurecount pipeline. Differential gene expression (FDR < 0.05) and gene ontology analysis was performed with edgeR and PathFindR.Results: Bulk analysis showed that at 1.5 days post AMI, R mice had significant lower expression of Hif1a (logFC: -1.24) and higher expression of Hif3a (logFC: 2.15) when compared to NR mice (p