EFFECT OF ADIPOSE MUSE CELL IMPLANTATION ON HOMING AND ANGIOGENESIS IN A SHEEP MODEL OF ACUTE MYOCARDIAL INFARCTION

CASTILLO M.G; PERALTA TM; LOCATELLI P; CROTTOGINI AJ; OLEA FD; CUNIBERTI LA

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAI&FAIC SAFIS 2022; 2022

SAIC, SAI, SAFIS

Objective Muse cells have been studied in a variety of diseases. However, the potential of Muse cells isolated from adipose tissue (AT) to promote new blood vessel formation in acute myocardial infarct (AMI) has not been entirely evaluated. The current study sought to investigate the neovascularization ability of AT-Muse cells in vitro and in vivo, as well as their differentiation potential in a translational ovine model of AMI. Methods Adipose-derived mesenchymal stromal cells (ASCs) and Adipose Muse cells were isolated from donor sheep. AMI was induced by thirty minutes of permanent ligation of one or more diagonal branches of the left anterior descending coronary artery. One hour after AMI, animals were randomized in two groups: 4 sheep received 10 intramyocardial injections of 2x107 AT-Muse cells labeled with PKH26 Red Fluorescent Dye and 4 sheep received PBS (Vehicle group). Results In vitro assay showed that the gene expression of angiogenic genes ANG, VEGF and PGF was higher in AT-Muse cells group in comparison with ASCs group, (p