Effects of meis1 overexpression in murine cardiomyocytes

LÓPEZ AE; BAUZÁ MDR ; SIMONIN JA; HALEK JM; CROTTOGINI A; BELAICH MN; OLEA FD; LOCATELLI P

Capital Federal

Congreso; Reunión Anual de la Sociedad Argentina de Fisiología; 2021

Sociedad Argentina de Fisiología

Introduction: As ischemic heart disease is the main cause of death worldwide there is particular interest in developing gene therapies to induce adult cardiomyocyte (CM) mitosis, an approach that would warrant efficient electromechanical coupling of new cells to the myocardial sincitium.The transcription factor Meis1 was proposed as a CM cell cycle inhibitor in mice, as its expression level increases from postnatal day 1 to P7, when CMs proliferative window is closed.Objectives: To transduce neonatal rat CMs with a baculoviral vector overexpressing meis1 (Bv.Meis1) and assess the effects on proliferation rate and the expression of angiogenic genes.Methods: Rat neonatal CMs were transduced with Bv.Meis1 at MOI 200. The effect of meis1 overexpression on cell proliferation was evaluated by cell count and MTS assay at 48 hours and 5 days post-transduction (PT). Angiogenic gene expression was assessed by RT-qPCR. Proliferation MTS assay was performed incubating HMEC cells with the supernatant of meis1 transfected CMs at the same time points. For statistical analysis we used t test (significance: p