INVESTIGADORES
OLEA Fernanda Daniela
artículos
Título:
Effect of intramyocardial administration of baculovirus encoding the transcription factor Tbx20 in sheep with experimental acute myocardial infarction
Autor/es:
BAUZÁ MDR; LÓPEZ AE; SIMONIN JA; CIMBARO FS; SCHARN A; CASTRO A; SILVESTRO CV; CUNIBERTI LA; CROTTOGINI AJ; BELAICH MN; LOCATELLI P; OLEA FD
Revista:
Journal of American Heart Association
Editorial:
John Wiley and Sons Inc
Referencias:
Lugar: Dallas; Año: 2024
ISSN:
2047-9980
Resumen:
BACKGROUND: Gene therapy has been proposed as a strategy to induce cardiac regeneration following acute myocardialinfarction (AMI). On account that Tbx20, a transcription factor of the T-box subfamily, stimulates cell proliferation and angiogenesis, we designed a baculovirus overexpressing Tbx20 (Bv-Tbx20) and evaluated its effects in cultured cardiomyocytesand in an ovine model of AMI.METHODS AND RESULTS: Cell proliferation and angiogenesis were measured in cardiomyocytes transduced with Bv-Tbx20 orBv-Null. Subsequently, in sheep with AMI, Bv-Tbx20 or Bv-Null were injected in the infarct border. Cardiomyocyte cell cycleactivity, angioarteriogenesis, left ventricular function, and infarct size were assessed. Cardiomyocyte-BvTbx20 increasedcell proliferation, cell cycle regulatory and angiogenic gene expression, and tubulogenesis. At 7days posttreatment, sheeptreated with Bv-Tbx20 showed increased Tbx20, promitotic and angiogenic gene expression, decreased levels of P21, increased Ki67- (17.09±5.73 versus 7.77±7.24cardiomyocytes/mm2, P
