MOLECULAR AND METABOLIC MECHANISM INVOLVED IN THE IMPARIMENT OF CD4 T CELL RESPONSE DURING TRYPANOSOMA CRUZI INFECTION

ANA, YAMILE; FOZZATTI, LAURA; ROJAS MARQUEZ, DAVID; BRUGO, MARIA BELEN; CERBAN, FABIO; STEMPIN, CINTHIA

Mar del Plata

Congreso; LXVI Reunión Anual de la Sociedad Argentina de Inmunología; 2018

Sociedad Argentina de Inmunología

We have previously shown an increased mitochondrial ROS (ROSm) production, mitochondrial alterations and a decreased glucose uptake in CD4 T cells during the acute phase of Trypanosoma cruzi infection. In order to determine the energy balance during infection we evaluated AMPK phosphorylation (p-AMPK) in CD4 T cells, purified from spleen of BALB/c mice infected with 500 trypomastigotes (tp) of Tulahuen strain at different times post infection (p.i) compared to CD4 T cells of control mice. CD4 T cells from acute phase of infection exhibit p-AMPK increase indicating unfavorable metabolic conditions. Besides, it has been demonstrated that CD11a and CD49d can be used as strategy to evaluate ?specific? CD4 T cells. We observed an increase in the frequency of the population CD4+CD11a+CD49d+ in infected mice (p