Inflammatory markers of radiation-induced late effects.

DUBNER D.; PORTAS M.; GALLEGOS C. E.; MICHELIN S.

Congreso; 14th International Congress of Radiation Research; 2011

Up to now there is no established parameters for the follow-up of delayed radiation injuries observed in victims of radiation accidents. Persistent inflammation has been associated with the late toxicity and pathological radiation fibrosis and it is well known that cellular adhesion molecules (CAMs) play a key role in the inflammatory process genesis. The following study was conducted to examine the response of the immune system in the inflammatory reactions through the expression of CAMs (CAM1 and a1-integrin) on granulocytes, lymphocytes and monocytes, as well as changes in subpopulations of lymphocytes in patients with skin injuries after radiotherapy or interventional fluoroscopy procedures. Were included in this research protocol twenty patients that showed reactions in tissues (skin, subcutaneous cellular tissue, muscle, etc.) by early and late toxicity due to medical exposures to ionizing radiation, graded according to the RTOG/EORTC system. In the samples, taken along the follow-up period, the expression of adhesion molecules was measured by staining with FITC-conjugated monoclonal mouse anti-human antibodies and lymphocyte subsets in whole blood were determined by Tritest (Becton Dickinson). Flow cytometry was performed on a Becton Dickinson FACS Calibur cytometer. A significant increase in the expression of a1-integrin on lymphocytes (7.35+/-2.69 vs 3.56+/-0.68, p