Mesenchymal stromal cells are modulated by thyroid hormona-responsive tumor microenviroment.

AMORÓS MARIANA; CAYROL FLORENCIA; GUITIERREZ LUCIANA; CERCHIETTI LEANDRO; CREMASCHI GRACIELA; BOLONTRADE MARCELA

Congreso; LXI Reunión Científica Anual de la Sociedad. Argentina de Investigación Clínica; 2016

Mesenchymal Stromal Cells (MSC) are recruited and homein the tumor stroma. Functional properties such as immunomodulation,migration and secretion of paracrine factors turn MSCas modulators of the tumor environment. Identifying the nichecomponents that modulate the cross talk between tumor cellsand MSC is key to understand progression mechanisms. Tumorenvironmental factors may affect MSC functions, influencing theirtumor modulation ability with an impact in tumor progression. Inthis regard thyroid hormones (TH) affect tumor growth. In orderto study the contribution of MSC to tumor development we usedour previously established model for T-cell lymphoblastic leukemiawith MSCs as recruited tumor stromal cell components, evaluatingthe modulation exerted by TH on MSC in a tumor environmentcontext. We demonstrated that TH-modulated tumor cells (canonicalpathway) produced a secretome that induced lower migrationrates and higher adhesiveness, together with an increased abilityto induce angiogenesis in vitro and a higher proliferation rate onMSC. When tumor cells were modulated by TH via surface recep -tors (non-canonical pathway) the resultant secretome inducedhigher migration rates (252,6±35,49 vs 168,9±9,22 No. of cells/field, non-canonical vs canonical respectively, p