Granulocyte colony-stimulating factor (G-CSF) upregulates β1 integrin and increases migration of human trophoblast Swan 71 cells via PI3K and MAPK activation.

FURMENTO VA; MARINO J; BLANK VC; CAYROL MF; CREMASCHI GA; AGUILAR RC

EXPERIMENTAL CELL RESEARCH

ELSEVIER INC

Lugar: Amsterdam ; Año: 2016

0014-4827

Exp Cell Res. 2016 Mar 15;342(2):125-34. doi: 10.1016/j.yexcr.2016.03.005. Epub 2016 Mar 15.Granulocyte colony-stimulating factor (G-CSF) upregulates β1 integrin and increases migration of human trophoblast Swan 71 cells via PI3K and MAPK activation.Furmento VA1, Marino J1, Blank VC1, Cayrol MF2, Cremaschi GA2, Aguilar RC3, Roguin LP4.Author informationAbstractMultiple cytokines and growth factors expressed at the fetal-maternal interface are involved in the regulation of trophoblast functions and placental growth, but the role of G-CSF has not been completely established. Based on our previous study showing that G-CSF increases the activity of matrix metalloproteinase-2 and the release of vascular endothelial growth factor in Swan 71 human trophoblast cells, in this work we explore the possible contribution of G-CSF to cell migration and the G-CSF-triggered signaling pathway. We found that G-CSF induced morphological changes on actin cytoskeleton consistent with a migratory cell phenotype. G-CSF also up-regulated the expression levels of β1 integrin and promoted Swan 71 cell migration. By using selective pharmacological inhibitors and dominant negative mutants we showed that PI3K, Erk 1/2 and p38 pathways are required for promoting Swan 71 cell motility. It was also demonstrated that PI3K behaved as an upstream regulator of Erk 1/2 and p38 MAPK. In addition, the increase of β1 integrin expression was dependent on PI3K activation. In conclusion, our results indicate that G-CSF stimulates β1 integrin expression and Swan 71 cell migration by activating PI3K and MAPK signaling pathways, suggesting that G-CSF should be considered as an additional regulatory factor that contributes to a successful embryo implantation and to the placenta development.Copyright © 2016 Elsevier Inc. All rights reserved.KEYWORDS:G-CSF; MAPK; Migration; PI3K; Swan 71 cells; β1 IntegrinPMID: 26992288 [PubMed - in process]Share on FacebookShare on TwitterShare on Google+