Multiple response optimization applied to the development of a ion paring liquid chromatographic method for the determination of nicarbazin in bulk and pharmaceutical dosage forms

TEGLIA CM; CÁMARA MS; ROBLES JC; DE ZAN MM

Córdoba

Congreso; 1º Reunión Internacional de Ciencias Farmacéuticas; 2010

Universidad Nacional de Córdoba

In developing a HPLC method, optimization may be applied to reduce the analysis time achieving desirable values for critical chromatographic parameters. The need of taking into account different aspects of the analysis at the same time calls for the use of multicriteria optimization. Experimental design, specifically surface response analysis, is a valuable tool in order to deal with this scope (1). In addition, when different objective functions have to be optimized, the so called ?Derringer?s desirability function? is a powerful strategy (2). Nicarbazin (NICA) is the generic name for the complex of 4,4`-dinitrocarbanilide (DNC) and 2-hydroxy-4,6-dimethylpyrimidine (HDP) in a 1:1 molar ratio, that is used as a feed additive against coccidiosis in poultry. No methodology was found in literature to the simultaneous assay of both components of NICA, possibly due to the bad retention of HDP in conventional RP-HPLC since it height polarity and basic properties. In this work an ion paring- high performance liquid chromatographic (IP-HPLC) method was developed, optimized and validated for its application in the determination of DNC and HDP in bulk material and coccidiostatic formulations.