Rapid determination of retinoic acid and its main isomers in plasma by second-order high-performance liquid chromatography data modeling

CARLA M. TEGLIA; MARÍA S. CÁMARA; HÉCTOR C. GOICOECHEA

ANALYTICAL AND BIOANALYTICAL CHEMISTRY

SPRINGER HEIDELBERG

Lugar: HEIDELBERG; Año: 2014 vol. 406 p. 7989 - 7998

1618-2642

This paper reports the development of a method based on high-performance liquid chromatography (HPLC) coupled to second-order data modeling with multivariate curve resolution-alternating least-squares (MCR?ALS) for quantification of retinoic acid and its main isomers in plasma in only 5.5 min. The compounds retinoic acid (RA), 13-cis-retinoic acid, 9-cis-retinoic acid, and 9,13-di-cis-retinoic acid were partially separated by use of a Poroshell 120 EC?C18 (3.0mm×30mm, 2.7 μm particle size) column. Overlapping not only among the target analytes but also with the plasma interferents was resolved by exploiting the second-order advantage of the multi-way calibration. A validation study led to the following results: trueness with recoveries of 98.5-105.9% for RA, 95.7-110.1% for 13-cis-RA, 97.1-110.8% for 9-cis-RA, and 99.5-110.9% for 9,13-di-cis-RA; repeatability with RSD of 3.5-3.1% for RA, 3.5-1.5% for 13-cis-RA, 4.6-2.7% for 9-cis-RA, and 5.2?2.7% for 9,13-di-cis-RA (low and high levels); and intermediate precision (inter-day precision) with RSD of 3.8-3.0% for RA, 2.9-2.4% for 13-cis-RA, 3.6-3.2% for 9,13-di-cis-RA, and 3.2-2.9% for 9-cis-RA (low and high levels). In addition, a robustness study revealed the method was suitable for monitoring patients with dermatological diseases treated with pharmaceutical products containing RA and 13-cis-RA