CHLORPYRIFOS AND CYPERMETHRIN INSECTICIDES INDUCE PROLIFERATION AND MIGRATION IN A HUMAN ENDOMETRIAL CANCER CELL LINE

GISELLE FAINBERG; MARIANELA LASAGNA; JOSÉ LUIS RANGEL; FLORENCIA CHIAPPINI; MARIANA MARDIROSIAN; CLAUDIA COCCA; MARIEL NÚÑEZ

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAB AAFE AACYTAL 2023; 2023

Chlorpyrifos (CPF) and cypermethrin (CPM) are insecticides widely used in our country, both individually and in combination. We previously demonstrated that CPF acts as an endocrine disruptor in rats inducing uterine endometrial cell proliferation. Our objective was to evaluate whether CPF, CPM or the combination of both of them affects human endometrial cancer ECC-1 cell proliferation and/or migration and to elucidate the role of estrogen receptor α (ERα) in these actions. ECC-1 cells were exposed to CPF or CPM environmental concentrations ranged from 0.05 to 50 µM. Clonogenic assays were performed in the presence or absence of the specific ERα inhibitor ICI 182,780 (ICI). To assess migration we used the wound healing assay. The estrogen receptor α (ESR-1) mRNA expression by qRT-PCR and cell cycle by flow cytometry were also studied. CPF and CPM at 0.05 µM induced proliferation (p