Proliferative effects of chlorpyrifos and cypermethrin on normal and tumoral human endometrium cell lines.

GISELLE FAINBERG; MARIANELA LASAGNA; JOSÉ LUIS RANGEL; FLORENCIA CHIAPPINI; CLARA VENTURA; MARIANA MARDIROSIAN; CLAUDIA COCCA; MARIEL NÚÑEZ

Mar del Plata

Congreso; Reunion Anual de Sociedades de Biociencias 2022; 2022

Chlorpyrifos (CPF) and cypermethrin (CPM) are two pesticides widely used in Argentina. We previously demonstrated that the pesticide CPF acts as an endocrine disruptor in rats inducing alteration of the estrous cycle and proliferation of uterine endometrial cells. The aim of this work was to evaluate whether CPF or CPM affect the proliferation and/or the viability of THESCs and ECC-1, normal and tumoral human endometrial cells, respectively. We also studied the role of estrogen receptor alpha (ER) in the pesticides action. THESCs and ECC-1 cell lines were exposed to CPF or CPM concentrations from 0.05 to 50 µM. Clonogenic assays were performed in the presence or absence of the specific ERα inhibitor (ICI 182,780). Cell viability by MTT assay and ER isoforms expression by western blot were also assessed. CPF and CPM at 0.05 µM induced proliferation in both cell lines (THESCs: p