BMP2, 4, 6 and BMPR1B are altered from early stages of bovine cystic ovarian disease development.
DÍAZ P.U.# EQUAL CONTRIBUTOR; HEIN G. J.# EQUAL CONTRIBUTOR; BELOTTI, M.; RODRIGUEZ F. M.; REY F.; AMWEG, A.; MATILLER, V.; BARAVALLE M. E.; ORTEGA H. H.; SALVETTI N.R.
Lugar: Bristol; Año: 2016
Cystic ovarian disease (COD) is an important cause of subfertility in dairy cattle. Bone Morphogenetic Proteins (BMPs), mainly BMP2, BMP4, and BMP6,play a key role in female fertility. In this study, we hypothesized that an alteredBMP system is associated with ovarian alterations contributing to CODpathogenesis. Therefore, we examined the expression of BMP2, BMP4, BMP6and BMP receptor 1B (BMPR1B) in the ovaries of animals with spontaneous orACTH-induced COD, as well as during the development of the disease, in amodel of follicular persistence induced by low doses of progesterone (at 5, 10and 15 days of follicular persistence). Results showed changes in BMP2, BMP4and BMP6 expression during folliculogenesis, in granulosa and theca cells inthe COD groups, as well as at the different stages of follicular persistence.Results also showed changes in BMPR1B expression in developing follicles inanimals with COD, and at the initial stages of follicular persistence (P5).Comparison between groups showed significant differences, mainly in BMP4and BMP6 expression, in granulosa and theca cells of different follicularcategories. The expression of these BMPs was also increased in cystic andpersistent follicles, in relation to antral follicles of the control group. BMPR1B showed high expression in cystic follicles. Together, these results may indicate an alteration in BMPs, especially in BMP4 and BMP6, as well as in BMPR1B, which occurs early in folliculogenesis and incipiently during the development of COD, and which could be a major cause of recurrence of this disease in cattle.