PREPARATION AND EVALUATION OF CIPROFLOXACIN DELIVERY FROM POLY(3-HYDROXYBUTYRATE) MEMBRANES

BERMUDEZ JOSÉ M; ROMERO ANÁLIA I; MERCEDES VILLEGAS; DIB ASHUR MARÍA FLORENCIA; PARENTIS MÓNICA L; CASTRO VIDAURRE E.F.

Rio de Janeiro

Simposio; II Simposio de Processos de Separacao com Membranas (SIMPAM 2013); 2013

PAM/PEQ/COPPE/UFRj

Polymeric membranes are being investigated and used in pharmaceutical technology as controlled release systems to modulate body drug release. In this study we have evaluated the potential application of novel membranes constituted of poly (3-hydroxybutyrate) (PHB). PHB is an intracellular polyester synthesized by certain bacteria as a carbon and energy storage compound and shows promising applications in medicine due to its biodegradability and biocompatibility. Ciprofloxacine hydrochloride (Cipro) was the model drug used for the release studies. Cipro is a second-generation fluoroquinolone antibiotic, valued for its broad spectrum of activity and excellent tissue penetration. Composite membranes loaded with 5 (PHB-Cipro-5) and 15%wt Cipro (PHB-Cipro- 15) were synthesized. Pure PHB polymeric membranes and PHB-Cipro loaded membranes were characterized using differential scanning calorimetry (DSC), electron scanning microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). Homogeneous composite membranes were obtained. FT-IR and DSC showed an interaction between Cipro and PHB, demonstrating that there is not only a physical mixture. SEM micrographs (Fig.1) provided evidences of a smooth and uniform structure. Cipro release was measured in three different media: water, saline solution and a pH 6.8 buffer solution. From release profiles, kinetic parameters and release mechanism were determined. It was observed that PHB membranes modulate Cipro release with appropriate release profiles; drug release was over 50% and followed Fickian release mechanisms according to the model applied for the kinetic analysis. These novel membranes are potentially useful platforms for formulating drug delivery systems.