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DORFMAN Damian

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Título:

Superior cervical gangliectomy induces dry age-related macular degeneration in mice: a new experimental model

Autor/es:

DIEGUEZ, HERNÁN H; ROMEO, HORACIO E; GONZÁLEZ FLEITAS, MARÍA F; ARANDA, MARCOS L; MILNE, GEORGIA A; ROSENSTEIN, RUTH E; DORFMAN, DAMIÁN

Lugar:

Buenos Aires

Reunión:

Congreso; Reunión Conjunta de Sociedades Biomédicas, LVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2017

Resumen:

Dry age-related macular degeneration, aprevalent cause of blindness, is a progressive and degenerative disease,characterized by alterations in Bruch´s membrane, retinal pigment epithelium,and photoreceptors exclusively localized in the macula. Despite there areexperimental murine models, the vast majority take too long to develop retinalalterations, which in general are ubiquitous, many result from non-eye specificgenetic manipulations, and most do not always reproduce the hallmarks of humanage-related macular degeneration. Choroid vessels receive sympatheticinnervation from the superior cervical ganglion, which together with theparasympathetic system, regulate the blood flow. Choroid blood flow changeshave been involved in age-related macular degeneration development andprogression. At present no experimental models take this factor into account.The aim of this work was to analyze the effect of superior cervicalgangliectomy on the choroid, Bruch´s membrane, retinal pigment epithelium, andretina. Adult male C57BL/6J mice were submitted to unilateral superior cervicalgangliectomy and a contralateral sham procedure. Although superior cervicalgangliectomy induced ubiquitous choroid and choriocapillaris changes(p<0.01), it induced Bruch´s membrane thickening, retinal pigment epitheliummelanin content and retinoid isomerohydrolase loss, drusen-like depositoccurrence, and retinal pigment epithelium and photoreceptors atrophy,exclusively localized in the temporal side (p<0.01). Moreover, superiorcervical gangliectomy provoked a localized increase in retinal pigmentepithelium and photoreceptors apoptosis (p<0.01), and photoreceptorselectroretinographic function decline (p<0.01). Therefore, superior cervicalgangliectomy recapitulated the main features of human dry age-related maculardegeneration, and could become a new experimental model of dry age-relatedmacular degeneration, and a useful platform for developing new therapies.

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